FISH Evolume™ ALK Break Apart Probe

Detection of ALK gene rearrangements by fluorescence in situ hybridization

The Evolume™ ALK Break Apart FISH probe was developed for the evaluation of rearrangements involving the ALK (Anaplastic Lymphoma Kinase) gene using the Fluorescence in situ Hybridization (FISH) technique.

Located in the 2p23.2 region of chromosome 2 , the ALK gene may be involved in chromosomal abnormalities associated with various neoplasms. The Break Apart configuration allows for the assessment of gene region integrity based on the distribution of fluorescent signals, aiding in the identification of rearrangements involving ALK.

Accuracy in the detection of ALK gene rearrangements

The probe uses two fluorescent markers located in distinct regions of the ALK gene:

• Orange probe: approximately 400 kb, targeting the 3′ region of ALK.
• Green probe: approximately 800 kb, targeting the 5′ region of ALK.

In cells without rearrangements, the fluorescent signals remain close together or overlap. Changes in the separation pattern of the signals may indicate the presence of rearrangements involving the ALK gene.

Clinical Application

ALK gene rearrangements, including the EML4-ALK fusion, are associated with non-small cell lung cancer (NSCLC).

FISH analysis allows for the investigation of these chromosomal abnormalities directly in the cells of the sample, contributing to the molecular characterization of the cases evaluated.

Break Apart Technology

The Break Apart strategy was developed to detect gene rearrangements without relying exclusively on the identification of a specific fusion partner.

The analysis is based on the behavior of two fluorescent probes positioned on opposite sides of the region of interest in the ALK gene.

Target gene
ALK — Anaplastic Lymphoma Kinase

Chromosomal location:
2p23.2

Technology
FISH — Fluorescent in situ hybridization

Probe configuration
Break Apart, dual color

Region 3′
Orange signal — approximately 400 kb

Region 5′
Green light — approximately 800 kb

Product information

Product: FISH Evolume™ ALK Break Apart Probe
Brand: Evolume™
Product Line: FISH Probes
Marketing in Brazil: Easypath
Target gene: ALK
Probe Type: Break Apart
Method: FISH
Healgen Reference Kits: 5, 10, and 20 tests
Storage: below -15 °C

The technical information regarding configuration, presentation, and storage is based on the ALK Break Apart FISH Probe Kit listed in the Healgen 2025 catalog.

Clearly and accurately identify ALK rearrangements

The Evolume™ ALK Break Apart FISH Probe combines dual-color fluorescent labeling and a Break Apart configuration to aid in the analysis of alterations involving one of the most clinically relevant genetic regions in the molecular characterization of non-small cell lung cancer.

Request an express quote and learn about the Evolume™ line of FISH probes.