{"id":20966,"date":"2025-11-19T17:40:03","date_gmt":"2025-11-19T20:40:03","guid":{"rendered":"https:\/\/easypath.com.br\/produto\/evolume-alk-break-apart-fish-probe\/"},"modified":"2026-11-10T07:55:47","modified_gmt":"2026-11-10T10:55:47","slug":"evolume-alk-break-apart-fish-probe","status":"publish","type":"product","link":"https:\/\/easypath.com.br\/en\/produto\/evolume-alk-break-apart-fish-probe\/","title":{"rendered":"Evolume\u2122 ALK Break Apart FISH Probe"},"content":{"rendered":"

FISH Evolume\u2122 ALK Break Apart Probe<\/h1>\n

Detection of ALK gene rearrangements by fluorescence in situ hybridization<\/h2>\n

The Evolume\u2122 ALK Break Apart FISH probe<\/strong> was developed for the evaluation of rearrangements involving the ALK (Anaplastic Lymphoma Kinase)<\/strong> gene using the Fluorescence in situ Hybridization (FISH)<\/strong> technique.<\/p>\n

Located in the 2p23.2 region of chromosome 2<\/strong> , the ALK gene may be involved in chromosomal abnormalities associated with various neoplasms. The Break Apart<\/strong> configuration allows for the assessment of gene region integrity based on the distribution of fluorescent signals, aiding in the identification of rearrangements involving ALK.<\/p>\n

\n

Accuracy in the detection of ALK gene rearrangements<\/h2>\n

The probe uses two fluorescent markers located in distinct regions of the ALK gene:<\/p>\n

    \n
  • Orange probe:<\/strong> approximately 400 kb<\/strong>, targeting the 3′<\/strong> region of ALK<\/strong>.<\/li>\n
  • Green probe:<\/strong> approximately 800 kb<\/strong>, targeting the 5′<\/strong> region of ALK<\/strong>.<\/li>\n<\/ul>\n

    In cells without rearrangements, the fluorescent signals remain close together or overlap. Changes in the separation pattern of the signals may indicate the presence of rearrangements involving the ALK gene.<\/p>\n

    \n

    Clinical Application<\/h2>\n

    ALK<\/strong> gene rearrangements, including the EML4-ALK<\/strong> fusion, are associated with non-small cell lung cancer (NSCLC)<\/strong>.<\/p>\n

    FISH analysis allows for the investigation of these chromosomal abnormalities directly in the cells of the sample, contributing to the molecular characterization of the cases evaluated.<\/p>\n

    \n

    Break Apart Technology<\/h2>\n

    The Break Apart<\/strong> strategy was developed to detect gene rearrangements without relying exclusively on the identification of a specific fusion partner.<\/p>\n

    The analysis is based on the behavior of two fluorescent probes positioned on opposite sides of the region of interest in the ALK gene.<\/p>\n

    Target gene<\/strong>
    \n ALK \u2014 Anaplastic Lymphoma Kinase<\/p>\n

    Chromosomal location<\/strong>:
    \n2p23.2<\/p>\n

    Technology<\/strong>
    \n FISH \u2014 Fluorescent in situ hybridization<\/p>\n

    Probe configuration<\/strong>
    \n Break Apart, dual color<\/p>\n

    Region 3′<\/strong>
    \n Orange signal \u2014 approximately 400 kb<\/p>\n

    Region 5′<\/strong>
    \n Green light \u2014 approximately 800 kb<\/p>\n

    \n

    Product information<\/h2>\n

    Product:<\/strong> FISH Evolume\u2122 ALK Break Apart Probe
    \n Brand:<\/strong> Evolume\u2122
    \n Product Line:<\/strong> FISH Probes
    \n Marketing in Brazil:<\/strong> Easypath
    \nTarget gene:<\/strong> ALK
    \nProbe Type:<\/strong> Break Apart
    \nMethod:<\/strong> FISH
    \nHealgen Reference Kits:<\/strong> 5, 10, and 20 tests
    \nStorage:<\/strong> below -15 \u00b0C<\/strong><\/p>\n

    The technical information regarding configuration, presentation, and storage is based on the ALK Break Apart FISH Probe Kit<\/strong> listed in the Healgen 2025 catalog.<\/p>\n

    \n

    Clearly and accurately identify ALK rearrangements<\/h1>\n

    The Evolume\u2122 ALK Break Apart FISH Probe<\/strong> combines dual-color fluorescent labeling and a Break Apart configuration to aid in the analysis of alterations involving one of the most clinically relevant genetic regions in the molecular characterization of non-small cell lung cancer.<\/p>\n

    Request an express quote and learn about the Evolume\u2122 line of FISH probes.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

      \n
    • Detection of ALK Gene Rearrangements<\/li>\n
    • Dual-color Break Apart FISH technology<\/li>\n
    • Clear visualization via fluorescent signals<\/li>\n
    • Applicable to molecular investigation of NSCLC<\/li>\n
    • Probes targeting the 3\u2019 and 5\u2019 regions of ALK<\/li>\n<\/ul>\n","protected":false},"featured_media":18008,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[974],"product_tag":[978,977,976,980,981,979],"class_list":["post-20966","product","type-product","status-publish","has-post-thumbnail","product_cat-evolume-fish-probes","product_tag-cytogenetics","product_tag-fish","product_tag-in-situ-hybridization","product_tag-oncology-and-hematology","product_tag-probe","product_tag-ruo","first","instock","shipping-taxable","purchasable","product-type-variable","col-xs-6 col-sm-4","col-md-3 col-lg-2","un-6-cols"],"_links":{"self":[{"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/product\/20966","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/comments?post=20966"}],"version-history":[{"count":0,"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/product\/20966\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/media\/18008"}],"wp:attachment":[{"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/media?parent=20966"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/product_brand?post=20966"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/product_cat?post=20966"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/easypath.com.br\/en\/wp-json\/wp\/v2\/product_tag?post=20966"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}