OncoTrace™ PMS2 (QR009) – Recombinant Antibody for Microsatellite Instability Diagnosis
High Reliability in Detecting MMR System Deficiencies
OncoTrace™ PMS2 (Clone QR009) is a recombinant rabbit monoclonal antibody (IgG), developed for the nuclear detection of the PMS2 protein in FFPE tissues. The PMS2 protein is an essential part of the DNA mismatch repair (MMR) system, acting in conjunction with MLH1. The absence of its expression is associated with microsatellite instability (MSI), a central feature in Lynch syndrome (HNPCC) and various tumors, including colorectal and endometrial cancer.
Why choose OncoTrace™ PMS2?
- Critical Marker: PMS2 is indispensable in the MMR panel (along with MLH1, MSH2 and MSH6) to screen for Lynch syndrome.
- High Diagnostic Reliability: Nuclear loss of PMS2 indicates deficiency of the MMR system and increased risk of MSI.
- Recombinant Production: Guarantees consistent and standardized results, eliminating variations between batches.
- Application in Various Tumors: Especially useful in colorectal cancer, endometrial cancer and other neoplasms associated with Lynch syndrome.
- Compatible with Automation: Validated for manual protocols and automated IHQ platforms.
- Cruelty Free: Produced in vitro, without the use of animals.
Diagnostic Applications
OncoTrace™ PMS2 helps diagnose deficiencies in the MMR system and is an essential part of assessing microsatellite instability. Its integrated use with the OncoTrace™ MLH1, MSH2 and MSH6 antibodies allows for greater precision in screening for Lynch syndrome and in diagnosing tumors with an MSI pattern.
Comparison: Recombinant vs. hybridoma
| Features | OncoTrace™ Recombinant | Traditional hybridoma |
|---|---|---|
| Origin | Produced in vitro (without animals) | Produced in mice |
| Reliability | Identical performance between batches | Frequent variation |
| Specificity | High affinity, minimal background | Higher risk of cross-reactivity |
| Standardization | Fully controlled | Subject to genetic drift |
Technical Specifications
- Clone: QR009
- Host: Rabbit (IgG)
- Immunogen: Synthetic peptide of the human protein PMS2
- Application: Immunohistochemistry (IHC) on FFPE tissues
- Cellular Location: Nuclear
- Recommended dilution (Concentrate): 1:20
- Positive Control: Amygdala (mantle zone and germinal center) or colon adenocarcinoma
- Available volumes:
- Concentrate: 0.1 ml, 1 ml
- Ready for use: 1 ml, 6 ml
- Storage: 2°C – 8°C (stable until expiration date)
Frequently Asked Questions (FAQ)
What is the OncoTrace™ PMS2 antibody used for?
It is used to assess microsatellite instability (MSI) and to screen for Lynch syndrome, as well as helping to diagnose colorectal and endometrial tumors.
What are the advantages of the recombinant format?
The recombinant format offers maximum standardization, reproducible results and elimination of batch-to-batch variability, increasing diagnostic reliability.
Is the product compatible with automation?
Yes. OncoTrace™ PMS2 is validated for use on automated platforms and in manual IHQ protocols.
How to store it?
Keep between 2°C and 8°C. Product stable until expiry date even after opening.
Confidence in High Complexity Diagnostics
Strengthen cancer screening with recombinant technology. Request your quote or sample of OncoTrace™ PMS2 (QR009) now.
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